研究成果

2022年の研究成果

永井 義隆（近畿大学・医学部・脳神経内科　教授）

  1. Fujino Y, *Nagai Y. The molecular pathogenesis of repeat expansion diseases. Biochem Soc Trans. 50(1):119-134 (2022) doi: 10.1042/BST20200143

松本有樹修（九州大学・生体防御医学研究所　准教授）

  1. †Mise S, *†Matsumoto A, Shimada K, Hosaka T, Takahashi M, Ichihara K, Shimizu H, Shiraishi C, Saito D, Suyama M, Yasuda T, Ide T, Izumi Y, Bamba T, Kimura-Someya T, Shirouzu M, Miyata H, Ikawa M, *Nakayama KI. Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis. Nat. Commun. 28;13(1):1071 (2022)

遠藤斗志也（京都産業大学・生命科学部　教授）

  1. Matsumoto S, Ono S, Shinoda S, Kakuta C, Okada S, Ito T, Numata T, Endo T. GET pathway mediates transfer of mislocalized tail-anchored proteins from mitochondria to the ER. J. Cell Biol. in press (2022) doi: 10.1083/jcb.202104076

岩川 弘宙（東京大学・定量生命科学研究所　講師）

  1. *Iwakawa HO and *Tomari Y. Life of RISC: Formation, action, and degradation of RNA-induced silencing complex. Mol Cell. Vol. 82(1), 30-43 (2022)

花田 耕介（九州工業大学大学院・情報工学研究院　教授）

  1. Oguchi R, Hanada K, Shimizu M, Mishio M, Ozaki H, Hikosaka K. Enhanced growth rate under elevated CO2 conditions was observed for transgenic lines of genes identified by intraspecific variation analyses in Arabidopsis thaliana Plant Molecular Biology 1-13 (2022)

2021年の研究成果

田口 英樹（東京工業大学・科学技術創成研究院・細胞制御工学研究センター　教授）

  1. *Chadani Y, Sugata N, Niwa T, Ito Y, Iwasaki S, *Taguchi H. Nascent polypeptide within the exit tunnel stabilizes the ribosome to counteract risky translation. EMBO J. Oct 20:e108299. (2021) doi: 10.15252/embj.2021108299.

  2. Miwa T, *Taguchi H. Novel self-regulation strategy of a small heat shock protein for prodigious and rapid expression on demand. 【Review】. Current Genetics (2021) in press

千葉 志信（京都産業大学・生命科学部　教授）

  1. Sakiyama K, Shimokawa-Chiba N, Fujiwara K and Chiba S. Search for translation arrest peptides encoded upstream of genes for components of protein localization pathways. Nucleic Acids Res. 49, 1550-1566 (2021) doi: 10.1093/nar/gkab024.

内藤 哲（北海道大学大学院・農学研究院　研究院）

  1. *Sotta N, Chiba Y, Miwa K, Takamatsu S, Tanaka M, Yamashita Y, *Naito S, *Fujiwara T. Global analysis of boron-induced ribosome stalling reveals its effects on translation termination and unique regulation by AUG-stops in Arabidopsis shoots. Plant J. (2021) in press doi: 10.1111/tpj.2021.15248

永井 義隆（近畿大学・医学部・脳神経内科　教授）

  1. Ishiguro A, Lu J, Ozawa D, Nagai Y, Ishihama A. ALS-linked FUS mutations dysregulate G-quadruplex-dependent liquid-liquid phase separation and liquid-to-solid transition. J Biol Chem. 297(5):101284 (2021) doi: 10.1016/j.jbc.2021.101284
  2. Mori K, Gotoh S, Yamashita T, Uozumi R, Kawabe Y, Tagami S, Kamp F, Nuscher B, Edbauer D, Haass C, Nagai Y, Ikeda M. The porphyrin TMPyP4 inhibits elongation during the noncanonical translation of the FTLD/ALS-associated GGGGCC repeat in the C9orf72 gene. J Biol Chem. 297(4):101120 (2021) doi: 10.1016/j.jbc.2021.101120
  3. Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, Ikawa M. Precise CAG repeat contraction in a Huntington’s Disease mouse model is enabled by gene editing with SpCas9-NG. Commun Biol. 4(1):771 (2021) doi: 10.1038/s42003-021-02304-w
  4. Ishiguro T, *Nagai Y, Ishikawa K. Insight into spinocerebellar ataxia type 31 (SCA31) From Drosophila model. Front Neurosci. 15:648133 (2021) doi: 10.3389/fnins.2021.648133
  5. Shibata T, Nagano K, Ueyama M, Ninomiya K, Hirose T, Nagai Y, Ishikawa K, Kawai G, Nakatani K. Small molecule targeting r(UGGAA)n disrupts RNA foci and alleviates disease phenotype in Drosophila model. Nat. Commun. 12(1), 236 (2021)
  6. Hervás R, Fernández-Ramírez M.D.C, Galera-Prat A, Suzuki M, Nagai Y, Bruix M, Menéndez M, Laurents D.V, Carrión-Vázquez M. Divergent CPEB prion-like domains reveal different assembly mechanisms for a generic amyloid-like fold. BMC Biol. 19(1), 43 (2021) doi: 10.1186/s12915-021-00967-9
  7. Minakawa E.N, Nagai Y. Protein aggregation inhibitors as disease-modifying therapies for polyglutamine diseases. Front【Review】 Neurosci. 15, 621996 (2021) doi: 10.3389/ fnins.2021.621996

森 康治（大阪大学大学院・医学系研究科・精神医学　助教）

  1. Mori K*, Gotoh S, Yamashita T, Uozumi R, Kawabe Y, Tagami S, Kamp F, Nuscher B, Edbauer D, Haass C, Nagai Y, Ikeda M. The porphyrin TMPyP4 inhibits elongation during the noncanonical translation of the FTLD/ALS-associated GGGGCC repeat in the C9orf72 gene. J Biol Chem. Oct;297(4):101120 (2021) doi: 10.1016/j.jbc.2021.101120. Epub 2021 Aug 25. PMID: 34450161

松本有樹修（九州大学・生体防御医学研究所　准教授）

  1. Nita A, *Matsumoto A, Tang R, Shiraishi C, Ichihara K, Saito D, Suyama M, Yasuda T, Tsuji G, Furue M, Katayama B, Ozawa T, Murata T, Dainichi T, Kabashima K, Hatano A, Matsumoto M, *Nakayama KI. A ubiquitin-like protein encoded by the “noncoding” RNA TINCR promotes keratinocyte proliferation and wound healing. PLoS Genet. 5;17(8):e1009686 (2021)
  2. Nita A, Muto Y, Katayama Y, Matsumoto A, Nishiyama M, Nakayama KI*. The autism-related protein CHD8 contributes to the stemness and differentiation of mouse hematopoietic stem cells. Cell Rep. 34(5), 108688 (2021)

遠藤斗志也（京都産業大学・生命科学部　教授）

  1. Takeda H, Tsutsumi A, Nishizawa T, Lindau C, Busto JV, Wenz L-S, Ellenrieder L, Imai K, Straub SP, Mossmann W, Qiu J, Yamamori Y, Tomii K, Suzuki J, Murata T, Ogasawara S, Nureki O, Becker T, Pfanner N, Wiedemann N, Kikkawa M, Endo T. Mitochondrial sorting and assembly machinery operates by β-barrel switching. Nature 590 (7844), 163-169 (2021)
  2. Shiino H, Furuta S, Kojima R, Kimura K, Endo T, Tamura Y. Phosphatidylserine fiux into mitochondria unveiled by organelle-targeted Escherichia coli phosphatidylserine synthase PssA. FEBS J. 288, 3285-3299 (2021) (Online published on Dec 7. 2020) doi: 10.1111/febs.15657

松本 雅記（新潟大学大学院・医歯学総合研究科　教授）

  1. Egami R, Kokaji T, Hatano A, Yugi K, Eto M, Morita K, Ohno S, Fujii M, Hironaka K, Uematsu S, Terakawa A, Bai Y, Pan Y, Tsuchiya T, Ozaki H, Inoue H, Uda S, Kubota H, Suzuki Y, Matsumoto M, Nakayama KI, Hirayama A, Soga T, *Kuroda S. Trans-omic analysis reveals obesity-associated dysregulation of inter-organ metabolic cycles between the liver and skeletal muscle. iScience (2021)
  2. Johmura Y, Yamanaka T, Omori S, Wang T, Sugiura Y, Matsumoto M, Suzuki N, Kumamoto S, Yamaguchi K, Hatakeyama S, Takami T, Yamaguchi R, Shimizu E, Ikeda K, Okahashi N, Mikawa R, Suematsu M, Arita M, Sugimoto M, Nakayama KI, Furukawa Y, Imoto S, Nakanishi M. Senolysis by glutaminolysis inhibition ameliorates various age-associated disorders. Science 371(6526): 265-70, (2021).

渡邉 力也（理化学研究所　主任研究員）

  1. Shinoda H, Taguchi Y, Nakagawa R, Makino A, Okazaki S, Nakano M, Muramoto Y, Takahashi C, Takahashi I, Ando J, Noda T, Nureki O, Nishimasu H, Watanabe R. Amplification-free RNA detection with CRISPR-Cas13. Commun Biol. 4(1), 476 (2021) doi: 10.1038/s42003-021-02001-8.

太田 元規（名古屋大学大学院・情報学研究科　教授）

  1. Takeda S, Koike R, Fujiwara I, Narita A, Miyata M, Ota M, Maéda Y. Structural Insights into the Regulation of Actin Capping Protein by Twinfilin C-terminal Tail. J. Mol. Biol. 433, 166891 (2021) doi: 10.1016/j.jmb.2021.166891
  2. Takeda S, Koike R, Nagae T, Fujiwara I, Narita A, Maéda Y, Ota M. Crystal structure of human V-1 in the apo form. Acta Cryst. F 77, 13-21 (2021) doi:10.1107/ S2053230X20016829

2020年の研究成果

田口 英樹（東京工業大学・科学技術創成研究院・細胞制御工学研究センター　教授）

  1. Konno H, Watanabe-Nakayama T, Uchihashi T, Okuda M, Zhu L, Noriyuki Kodera, Kikuchi Y, *Ando T, *Taguchi H. Dynamics of oligomer and amyloid fibril formation by yeast prion Sup35 observed by high-speed atomic force microscopy. Proc Natl Acad Sci USA 117, 7831-7836 (2020)
  2. Miwa T, Chadani Y, *Taguchi, H. Escherichia coli small heat shock protein IbpA is an aggregation-sensor that self-regulates its own expression at post-transcriptional levels. Mol Microbiol 115, 142-156 (2020)
  3. Muta M, *Iizuka R, Niwa T, Guo Y, Taguchi H and *Funatsu T. Nascent SecM chain interacts with outer ribosomal surface to stabilize translation arrest. Biochem J. 477, 557-566 (2020)

千葉 志信（京都産業大学・生命科学部　教授）

  1. Fujiwara K, Katagi Y, Ito K and Chiba S. Proteome-wide Capture of Co-translational Protein Dynamics in Bacillus subtilis Using TnDR, a Transposable Protein-Dynamics Reporter. Cell Rep. 33, 108250 (2020) doi: 10.1016/j.celrep.2020.108250.

内藤 哲（北海道大学大学院・農学研究院　研究院）

  1. Takamatsu S, Ohashi Y, Onoue N, Tajima Y, Imamichi T, Yonezawa S, Morimoto K, Onouchi H, Yamashita Y and Naito S. Reverse genetics-based biochemical studies of the ribosomal exit tunnel constriction region in eukaryotic ribosome stalling: spatial allocation of the regulatory nascent peptide at the constriction. Nucleic Acids Res. 48, 1985-1999 (2020)
  2. *Takahashi H, Miyaki S, Onouchi H, Motomura T, Idesako N, Takahashi A, Murase M, Fukuyoshi S, Endo T, Satou K, Naito S, *Itoh M. Exhaustive identification of conserved upstream open reading frames with potential translational regulatory functions from animal genomes. Sci. Rep. 10, 16289 (2020). doi: 10.1038/s41598-020-73307-6
  3. *Takahashi H, Hayashi N, Hiragori Y, Sasaki S, Motomura T, Yamashita Y, Naito S, Takahashi A, Fuse K, Satou K, Endo T, Kojima S, *Onouchi H. Comprehensive genomewide identification of angiosperm upstream ORFs with peptide sequences conserved in various taxonomic ranges using a novel pipeline, ESUCA. BMC Genomics 21, 260 (2020) doi: 10.1186/s12864-020-6662-5

永井 義隆（近畿大学・医学部・脳神経内科　教授）

  1. Minakawa E.N, Popiel H.A, Tada M, Takahashi T, Yamane H, Saitoh Y, Takahashi Y, Ozawa D, Takeda A, Takeuchi T, Okamoto Y, Yamamoto K, Suzuki M, Fujita H, Ito C, Yagihara H, Saito Y, Watase K, Adachi H, Katsuno M, Mochizuki H, Shiraki K, Sobue G, Toda T, Wada K, Onodera O, *Nagai Y. Arginine is a disease modifier of polyQ disease models that stabilizes polyQ protein conformation. Brain 143(6), 1811-1825 (2020)
  2. Araki K, Yagi N, Ikemoto Y, Hayakawa H, Fujimura H, Moriwaki T, Nagai Y, Murayama S, Mochizuki H. The secondary structural differences between Lewy body and glial cytoplasmic inclusion in autopsy brain with synchrotron FTIR micro-spectroscopy. Sci. Rep. 10(1), 19423 (2020) doi:10.1038/s41598-020-76565-6

森 康治（大阪大学大学院・医学系研究科・精神医学　助教）

  1. Kawabe Y, Mori K, Yamashita T, Gotoh S, Ikeda M. The RNA exosome complex degrades expanded hexanucleotide repeat RNA in C9orf72 FTLD/ALS. EMBO J. 39(19), e102700(2020) doi: 10.15252/embj.2019102700. Epub 2020 Aug 24.

松本有樹修（九州大学・生体防御医学研究所　准教授）

  1. Kito Y, Matsumoto M, Hatano A, Takami T, Oshikawa K, Matsumoto A, Nakayama KI*. Cell cycle-dependent localization of the proteasome to chromatin. Sci. Rep. 10, 5801 (2020)

遠藤斗志也（京都産業大学・生命科学部　教授）

  1. Watanabe Y, Tamura Y, Kakuta C, Watanabe S, Endo T. Structural basis for inter-organelle phospholipid transport mediated by VAT-1. J Biol Chem. 295, 3257-3268 (2020)
  2. Araiso Y, Imai K, Endo T. Structural snapshot of the mitochondrial protein import gate 【Review】. FEBS J. online published on Dec 10, 2020 doi: 10.1111/febs.15661
  3. Shiino H, Furuta S, Kojima R, Kimura K, Endo T, Tamura Y. Phosphatidylserine fiux into mitochondria unveiled by organelle-targeted Escherichia coli phosphatidylserine synthase PssA. FEBS J. 288, 3285-3299 (2021) (Online published on Dec 7. 2020) doi: 10.1111/febs.15657

松本 雅記（新潟大学大学院・医歯学総合研究科　教授）

  1. Kokaji T, Hatano A, Ito Y, Yugi K, Eto M, Morita K, Ohno S, Fujii M, Hironaka KI, Egami R, Terakawa A, Tsuchiya T, Ozaki H, Inoue H, Uda S, Kubota H, Suzuki Y, Ikeda K, Arita M, Matsumoto M, Nakayama KI, Hirayama A, Soga T, *Kuroda S. Transomics analysis reveals allosteric and gene regulation axes for altered hepatic glucose-responsive metabolism in obesity. Sci. Signal. 13(660), eaaz1236 (2020)
  2. Tsukiyama T, Zou J, Kim J, Ogamino S, Shino Y, Masuda T, Merenda A, Matsumoto M, Fujioka Y, Hirose T, Terai S, Takahashi H, Ishitani T, Nakayama KI, Ohba Y, Koo BK. and Hatakeyama S. A phospho-switch controls RNF43-mediated degradation of Wnt receptors to suppress tumorigenesis. Nature Commun. 4586 (2020)
  3. Fang Y, Akimoto M, Mayanagi K, Hatano A, Matsumoto M, Matsuda S, Yasukawa T, Kang D. Chemical acetylation of mitochondrial transcription factor A occurs on specific lysine residues and affects its ability to change global DNA topology. Mitochondrion 53, 99-108 (2020)
  4. Nakagawa T, Hattori S, Nobuta R, Kimura R, Nakagawa M, Matsumoto M, Nagasawa Y, Funayama R, Miyakawa T, Inada T, Osumi N, Nakayama KI, Nakayama K. The AutismRelated Protein SETD5 Controls Neural Cell Proliferation through Epigenetic Regulation of rDNA Expression. iScience 23(4), (2020)
  5. Kito Y, *Matsumoto M, Hatano A, Takami T, Oshikawa K, Matsumoto A, *Nakayama KI. Cell cycle-dependent localization of the proteasome to chromatin. Sci. Rep. 10(1), 5801 (2020)
  6. Oshikawa K, *Matsumoto M, Kodama M, Shimizu H, *Nakayama KI. A failsafe system to prevent oncogenesis by senescence is targeted by SV40 small T antigen. Oncogene 39(10), 2170-86 (2020)

渡邉 力也（理化学研究所　主任研究員）

  1. Sakamoto S, *Komatsu T, *Watanabe R, Zhang Y, Inoue T, Kawaguchi M, Nakagawa H, Ueno T, Okusaka T, Honda K, *Noji H & *Urano Y. Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins inbiological samples. Sci Adv. 6, eaay0888 (2020)

太田 元規（名古屋大学大学院・情報学研究科　教授）

  1. Koike R, Amano M, Kaibuchi K, Ota M. Protein kinases phosphorylate long disordered regions in intrinsically disordered proteins. Prot. Sci. 29, 564-571 (2020) doi: 10.1002/pro.3789

福地 佐斗志（前橋工科大学・工学部　教授）

  1. Anbo H, Amagai H and Fukuchi S. NeProc predicts binding segments in intrinsically disordered regions without learning binding region sequences. Biophys. Physicobiol. 17, 147-154 (2020)